Project

Part:BBa_K3646009:Design

Designed by: Sukanya Chakraborty   Group: iGEM20_IISER_Berhampur   (2020-10-25)


FRaPPeT3:hSTAT2_CID_FRET


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4258
    Illegal PstI site found at 1720
    Illegal PstI site found at 1948
    Illegal PstI site found at 2296
    Illegal PstI site found at 3607
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4258
    Illegal PstI site found at 1720
    Illegal PstI site found at 1948
    Illegal PstI site found at 2296
    Illegal PstI site found at 3607
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4258
    Illegal BamHI site found at 1998
    Illegal BamHI site found at 2144
    Illegal BamHI site found at 3297
    Illegal XhoI site found at 1696
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4258
    Illegal PstI site found at 1720
    Illegal PstI site found at 1948
    Illegal PstI site found at 2296
    Illegal PstI site found at 3607
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4258
    Illegal PstI site found at 1720
    Illegal PstI site found at 1948
    Illegal PstI site found at 2296
    Illegal PstI site found at 3607
    Illegal NgoMIV site found at 3461
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3792


Design Notes

Gene synthesized using Splicing by Overlap Extension. Used as a part of FRaPPe combined CID-FRET reporter to study host-viral PPIs in mammalian HEK293 cells. Other components sourced from Addgene Plasmid 20160 https://www.addgene.org/20160/


Source

Gene sequence of hSTAT2 was sourced from https://www.ncbi.nlm.nih.gov.

GenBank Accession : U18671.1

eCFP and FKBP CDS is a part of the CF plasmid from Addgene used for designing the FRaPPe test construct T3.

References